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Tth Inorganic Pyrophosphatase (thermostable) Online Order

Tth Inorganic Pyrophosphatase (thermostable)

Native, thermostable Tth Inorganic Pyrophosphatase is a hydrolase purified from Thermus thermophilus. Tth Inorganic Pyrophosphatase catalyzes the conversion of inorganic pyrophosphate to orthophosphate in reactions where pyrophosphate is accumulated. These include DNA synthesis and amplification where Tth Inorganic Pyrophosphatase provides enhanced polymerisation, by removing inhibiting pyrophosphates in the reaction.

APPLICATIONS: 

  • enhanced DNA synthesis and amplificationl
  • longer fragments to be processedl
  • DNA sequencingl
  • in vitro mutagenesis
USE:

As PCR* is inhibited by the presence of pyrophosphate even at very low concentrations. The introduction of Tth Inorganic Pyrophosphatase into the reaction mixture greatly enhances amplification and provides superior results. The addition of 1 unit Tth Inorganic Pyrophosphatase to 10 units of thermostable DNA polymerase can double the level of PCR* amplification. Moreover Tth Inorganic Pyrophosphatase enables longer DNA fragments to be processed successfully and provides increased fidelity.

     

STORAGE BUFFER:

Incubate at 37oC. 10 mM K-phosphate buffer pH 7.0, 100 mM NaCl, 0.5 mM EDTA, 1 mM DTT, 0.01% Tween 20 and 50% glycerol (v/v).

 COMMENTS:

    Purified free of ATPase and phosphatase activities; quality control tests : activity, SDS/PAGE purity.
CONCENTRATION:

100 units/µl

 

     

 UNIT DEFINITION

    One unit is defined as the amount of enzyme required to convert 1µmole of pyrophosphate into 2µmoles of orthophosphate in one minute at 75°C under the following conditions : 1 mM K4P2O7, 2 mM MgCl2, 50 mM Tris-HCl (pH 9.0) at 75°C.

 STORAGE AND SHIPPING:

    store at –20°C. Shipped at room temperature.

    *PCR is a technology covered by patents owned by Hoffmann-La Roche Inc. and F. Hoffmann-La Roche Ltd.

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