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TAQ DNA Polymerase Online Order

TAQ DNA POLYMERASE- Licensed for PCR*

GENAXIS Biotechnology Taq DNA Polymerase is the recombinant form of the enzyme from the thermophilic eubacterium Thermus aquaticus BM expressed in E. coli. It is a highly processive 5´ -> 3´ DNA Polymerase, lacking 3´ -> 5´exonuclease activity. The high processivity and fidelity of GENAXIS Biotechnology Taq DNA Polymerase allows amplification of DNA fragments up to 8 kb. GENAXIS Biotechnology Taq DNA Polymerase is delivered with a 10X reaction buffer tube and a dilution buffer tube.

APPLICATIONS:

  • amplification of DNA fragments by PCR*
  • DNA sequencing
  • labeling of nucleic acids by PCR* with modified nucleotides
CONCENTRATION:

    5units/µl

REACTION BUFFER 10X:

    100mM Tris-HCl pH8.8, 500mM KCl, 1.0% Triton X-100. A tube of 25mM MgCl2 is provided separately.

 UNIT DEFINITION:

    one unit is defined as the amount of enzyme required to incorporate 10nmoles of dNTPs into an acid precipitable form in 30 minutes at 70ˇC.

COMMENTS:

    purified free of endonucleases ; good performance was confirmed by using Lambda DNA as template (amplified fragment 7.5kb) and human placenta DNA as template (amplified fragment 3.0kb).

 STORAGE BUFFER:

    20mM Tris-HCl pH8.0, 100mM KCl, 0.2mM EDTA, 1mM DTT, 55% (v/v) glycerol and stabilizers. If necessary the enzyme may be diluted before use. The diluted enzyme may be stored at Đ20ˇC up to six months.

 STORAGE AND SHIPPING:

    store at -20 oC,
    shipped at room temperature.

 

* PCR is a technology covered by patents owned by Hoffmann-La Roche Inc. and F. Hoffmann-La Roche Ltd.

Use of the PCR process requires a license. This product does not provide a license to use PCR.

The purchase price of this products includes limited, non-transferable rights under U.S. Patents
4,683,202, 4,683,195 and 4,965,188 and their foreign counterparts, owned by Roche Molecular Systems,
Inc. and F. Hoffmann-La Roche Ltd, to use only this amount of the product to practice the Polymerase
Chain Reaction ("PCR") Process described in said patents solely for the HLA Typing applications of the
purchaser solely for organ or tissue or bone marrow transplantation and explicitly excludes analysis of forensic
evidence and par-entage determination. The right to use this product to perform and to offer commercial
services for HLA Typing for organ or tissue transplantation using PCR, including reporting the results of the
purchaser's activities for a fee or other commercial consideration, is also hereby granted. Further information on
purchasing licenses to practice PCR may be obtained by contacting, in the United States, the Licensing
Department at Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, California 94501, and outside
the United States, the PCR Licensing Manager, F. Hoffmann-La Roche Ltd, Grenzacher-str. 124,
CH-4070 Basel, Switzerland.

For Research Use Only. This product has not been validated for diagnostic purposes. Diagnostic use of results
will stay in the sole responsibility of user. This product is optimised for use in Polymerase Chain Reaction (PCR)
process which is covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-LaRoche AG.
No license under these patents to use the PCR Process is conveyed expressly or by implication to the purchaser
by the pur-chase of this product. For further information on purchasing licenses to prac-tice the PCR Process
please contact the ROCHE Licensing Department.

For further information and prizes about these products send us an eMail or use our online order forms.