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Pwo DNA Polymerase Kit Online Order

Pwo DNA Polymerase Kit

Licensed for PCR*
Pwo DNA Polymerase is a highly processive 5’ ½ 3’ DNA polymerase with additional 3’ ½ 5’ exonuclease (proof-reading) activity. Pwo DNA Polymerase was originally isolated from the thermophilic archaebacterium Pyrococcus woesei; GENAXIS™ Biotechnology Pwo DNA Polymerase is the recombinant form cloned in E. coli. The enzyme has no detectable 5’ ½ 3’ exonuclease activity. Pwo DNA Polymerase exhibits increased thermal stability as well as a tenfold increase in fidelity of DNA synthesis compared to Taq DNA Polymerase. Pwo DNA Polymerase generates blunt-ended PCR* products.

APPLICATIONS:  

  • all standard PCR* reactions replacing Taq DNA Polymerasel
  • cloning of PCR* products (Pwo DNA Polymerase generates blunt-ended fragments)l
  • characterization of rare mutations in tissuel
  • characterization of allelic polymorphismsl
  • characterization of a cell population in culturel
  • labeling of nucleic acids by PCR* with modified nucleotides
KIT CONTENT:

    • 100 units Pwo DNA Polymerase     (100µl)
    • 10X reaction buffer complete     (1ml)
    • 10X reaction buffer incomplete     (1ml)
    • 25mM MgSO4     (1ml)
    • Dilution buffer     (1ml)

CONTENTRATION:

    1 unit/µl.

 REACTION BUFFER 10X COMPLETE:

100mM Tris-HCl pH8.8, 50mM (NH4)2SO4, 250mM KCl, 20mM MgSO4.

 

     
REACTION BUFFER 10X INCOMPLETE:

    100mM Tris-HCl pH8.8, 50mM (NH4)2SO4, 250mM KCl, without MgSO4. A tube of 25mM MgSO4 is provided separately.

 UNIT DEFINITION:

    one unit is defined as the amount of enzyme required to incorporate 10nmoles of dNTPs into an acid precipitable form after 30 minutes at 70°C.

 COMMENTS:

    purified free of endonucleases ; performance was confirmed by using Lambda DNA as template (amplified fragment 7.5kb) and human placenta DNA as template (amplified fragment 3.0kb). Optimization efforts are often required.
STORAGE AND DILUTION BUFFER:

20mM Tris-HCl
pH7.5, 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% NP 40, 0.5% Tween 20, 55% (v/v) glycerol and stabilizers. If necessary the enzyme can be diluted before use. Diluted enzyme can be stored at –20°C up to six months.

  

 

 STORAGE AND SHIPPING:

store at –20°C. Shipped at room temperature.

* PCR is a technology covered by patents owned by Hoffmann-La Roche Inc. and F. Hoffmann-La Roche Ltd.

Use of the PCR process requires a license. This product does not provide a license to use PCR.

The purchase price of this products includes limited, non-transferable rights under U.S. Patents
4,683,202, 4,683,195 and 4,965,188 and their foreign counterparts, owned by Roche Molecular Systems,
Inc. and F. Hoffmann-La Roche Ltd, to use only this amount of the product to practice the Polymerase
Chain Reaction ("PCR") Process described in said patents solely for the HLA Typing applications of the
purchaser solely for organ or tissue or bone marrow transplantation and explicitly excludes analysis of forensic
evidence and par-entage determination. The right to use this product to perform and to offer commercial
services for HLA Typing for organ or tissue transplantation using PCR, including reporting the results of the
purchaser's activities for a fee or other commercial consideration, is also hereby granted. Further information on
purchasing licenses to practice PCR may be obtained by contacting, in the United States, the Licensing
Department at Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, California 94501, and outside
the United States, the PCR Licensing Manager, F. Hoffmann-La Roche Ltd, Grenzacher-str. 124,
CH-4070 Basel, Switzerland.

For Research Use Only. This product has not been validated for diagnostic purposes. Diagnostic use of results
will stay in the sole responsibility of user. This product is optimised for use in Polymerase Chain Reaction (PCR)
process which is covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-LaRoche AG.
No license under these patents to use the PCR Process is conveyed expressly or by implication to the purchaser
by the pur-chase of this product. For further information on purchasing licenses to prac-tice the PCR Process
please contact the ROCHE Licensing Department.

For further information and prizes about these products send us an eMail or use our online order forms.