NUCLEIC_ACID
EXTRACTION
REAGENTS		 PCR & RELATED
PRODUCTS		 MODIFYING
ENZYMES		 ELECTRO-
PHORESIS		 CUSTOM
SERVICES
Isolation of Total RNA Isolation of DNA Isolation of Total
RNA/DNA/Proteins		 RNA/DNA Isolation Reagents

Liquid Phenol

stabilized
Cat# Packsize Description Product composition
12606
12608


 100 ml
500 ml


 Phenol water saturated
Phenol saturated with water; stabilized with 0.1% 8-Hydroxyquinoline; pH (20°C) : approx. 4.0; heavy metals : max. 0.0005%; water : approx. 9.0%.		 pH 4.2-5
12597
12599		 100 ml
500 ml		 Phenol Tris saturated
Phenol extracted with Tris-EDTA buffer; stabilized with 0.1% 8-Hydroxyquinoline; pH (20°C) : 7.6-8.0; heavy metals : max. 0.0005%; water : approx. 9.0%.		 pH 7.6-8.0
12627
12629		 100 ml
500 ml		 Phenol equilibrated,Chloroform/Isoamyl alcohol 25:24:1Liquid
Composition : 500 ml phenol equilibrated with Tris, stabilized with 0.1% 8-Hydroxyquinoline - 480 ml chloroform - 20 ml isoamyl alcohol; pH (20°C) : 7.6-8.0; heavy metals : max. 0.0005%; chloride : max. 0.0005%; water : approx. 9.0%.		 pH 7.6-8.0
non stabilized
Cat# Packsize Description Product composition
12600
12602		 100 ml
500 ml		 Phenol water saturated
Phenol saturated with water; without 8-Hydroxyquinoline; pH (20°C) : approx. 4.0; heavy metals : max. 0.0005%; water : approx. 9.0%.		 pH 4.0
12608
12605		 100 ml
500 ml		 Phenol water saturated,  separate Tris buffer for pH adjustment
Phenol saturated with water without 8-Hydroxyquinoline delivered with a separate Tris buffer (50 ml,250 ml for 100 ml, 500 ml phenol solution respectively); by adding different quantities of the Tris buffer, corresponding pH values (pH 6.5 - 8.0) can be adjusted before use (see example on the table); pH without adjustment : approx. 4.0; heavy metals : max. 0.0005%; water : approx. 9.0%.		 Phenol           starting volume                                       Tris buffer added  Final pH  value obtained  
100 ml   5 ml 6.50±0.1
100 ml 10 ml 6.88±0.1
100 ml 15 ml 7.15±0.1
100 ml 20 ml 7.35±0.1
100 ml 25 ml  7.48±0.1
12615
12617		 100 ml
500 ml		 Phenol water saturated,Chloroform/Isoamyl alcohol 25:24:1 + separate Tris buffer for pH adjustment Phenol           starting volume                Tris buffer added Final pH value obtained
100 ml   5 ml 7.38±0.1
100 ml 10 ml 7.59±0.1
100 ml 15 ml 7.76±0.1
100 ml 20 m 57.87±0.1
100 ml 25 ml   7.96±0.1

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