EXTRACTION
REAGENTS
PRODUCTS
ENZYMES
PHORESIS
SERVICES
RNA/DNA/Proteins
RNA/DNA Isolation Reagents
| ß-Agarase | ||
| From Pseudomonas atlantica Key facts :
|
ß-agarase is used for the recovery of pure DNA and RNA from agarose low melting gels (Ishimatsu K. et al. (1956) Sci. and Ind. (Japan), 30, 137) . One b-agarase unit will solubilize 500 mg of low melting agarose gel at 40-42°C within one hour. To isolate nucleic acids from the agarose gel, cut out the band from the gel as accurately as possible; add to the slice half of the volume (or weight) of 1X reaction buffer and melt the agarose at 40-42°C. Add one unit b-agarase per 500µl melted agarose and allow to incubate at 40-42° for one hour. Nucleic acids can then be recovered by a simple ethanol precipitation. | Storage and
Shipping: ß-agarase should be stored at -20°C; shipped refrigerated. |
|
||
| DEPC | ||
| Diethylpyrocarbonate | ||
| Storage: store at 2-8°C. |
||
|
||
| Guanidine Hydrochloride | ||
| Guanidinium chloride molecular biology grade | ||
| Storage: store at room temperature. |
||
|
||
| Guanidine Isothiocyanate | ||
| Guanidine Thiocyanate, Guanidine Rhodanide molecular biology grade | ||
| Storage: store at room temperature. |
||
|
||
| Lithium Chloride | ||
| Analytical grade | ||
| Storage: store at room temperature. |
||
|
||
| Phenol | ||
| Carbolic acid molecular biology grade | ||
| Storage: store at room temperature, in a dry place. |
||
|
||
| Phenol water saturated, stabilized | ||
| Liquid molecular biology grade | ||
| Storage: store at 2-8°C away from light (bottled under argon). |
||
|
||
| Phenol water saturated, non stabilized | ||
| Liquid molecular biology grade | ||
| Storage: store at 2-8°C away from light (bottled under argon). |
||
|
||
| Phenol tris saturated (pH 7.6-8.0), stabilized | ||
| Liquid molecular biology grade | ||
| Storage: store at 2-8°C away from light (bottled under argon). |
||
|
||
| Phenol water saturated, non stabilized + separate tris buffer for pH adjustment | ||
| Liquid molecular biology grade | ||
| Storage: store at 2-8°C away from light (bottled under argon). |
||
|
||
| Phenol equilibrated (pH 7.6-8.0), stabilized/chloroform/isoamyl, alcohol 25:24:1 | ||
| Liquid molecular biology grade | ||
| Storage: store at 2-8°C away from light (bottled under argon). |
||
|
||
| Phenol water saturated, non stabilized/chloroform/isoamyl, alcohol 25:24:1 + separate tris buffer for pH adjustment | ||
| Liquid molecular biology grade | ||
| Storage: store at 2-8°C away from light (bottled under argon). |
||
|
||
| RNase-EX | ||
| RNase decontamination solution | ||
| Storage and Shipping: RNase-Ex should be stored at room |
||
For further information and prizes about these products send us an eMail or use our online order forms.