NUCLEIC_ACID
EXTRACTION
REAGENTS
PCR & RELATED
PRODUCTS
MODIFYING
ENZYMES
ELECTRO-
PHORESIS
CUSTOM
SERVICES
Isolation of Total RNA Isolation of DNA Isolation of Total
RNA/DNA/Proteins
RNA/DNA Isolation Reagents

Isolation of DNA

GENExis
Kit for the isolation of genomic DNA from various biological samples Flow Diagram GENExis procedure for whole blood
Key facts :
  • complete kit
  • 45 minute procedure
  • for all types of biological samples
  • high yields
  • reproducible

Incubation of 1 vol. whole blood with 2 vol. Buffer 1 (5 min)

Chloroform extraction (vol/vol) and centrifugation (5 min)

Addition of Buffer 2
and DNA precipitation (15 min)

Resuspension of pellet in Buffer 3

Second DNA precipitation (15 min)

Resuspension of genomic DNA pellet in TE buffer


Undegraded genomic DNA was purified from human placenta tissue using GENExis
(lane 2).
Lane 1 : GenSizer II
Lane 3 : uncut l DNA (48 kbp
)
DNAxis
Kit for the isolation of DNA from solutions or agarose gels Flow Diagram DNAxis™ procedure/Agarose fragment Flow Diagram DNAxis™ procedure/DNA Solution
Key facts :
  • complete kit
  • 30 minute procedure
  • for agarose gels or solutions
  • phenol and guanidinium salts free
  • high recovery yields for small (>120bp) and large fragments (>20kb)
  • cost effective
Agarose fragment

Add solution 1 and heat at 55oC

Add the silica suspension

Incubate and allow to sediment

Wash pellet

Elute DNA
DNA solution

Add solution 1

Add the silica suspension

Incubate and allow to sediment

Wash pellet

Elute DNA

For further information and prizes about these products send us an eMail or use our online order forms.