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Custom Services
for oligonucleotide synthesis and DNA sequencing

| CUSTOM
OLIGONUCLEOTIDE SYNTHESIS |
| GENAXIS
Biotechnology offers high-quality, fast, custom synthesis and
modification of oligonucleotides. Both our industrial production facility
and expertise allow the synthesis of 5bp to more than 140bp in length
without additional cost. GENAXIS Biotechnology is also able to synthesize
crude or modified oligonucleotides in scales comprised between 10
nmoles to 10µmoles.
Quality: The quality and sequence of every
oligonucleotide is guaranteed. Each oligonucleotide is deprotected,
desalted and lyophilized so that it can be readily dissolved in
water or buffer. Upon request we may deliver your oligonucleotides
already solubilized in purified water or TE buffer. With each oligonucleotide
is also delivered a certificate stating : - sequence synthetized,
- name of the sequence, - GC ratio, - molar extinction coefficient,
- quantity delivered in OD, nmoles and µg - µl water or buffer necessary
to obtain a 50µM solution - Tm value
Pricing: Prices for standard or cartridge
purified oligonucleotides are set per base, with no set-up charges,
mixed site charges or hidden fees. For modification, labeling or
purification by PAGE or HPLC a set-up price is given.
To
order: You will find a special order
form here. When completed please return it by mail or contact
your local
distributor. If you need further technical assistance do not
hesitate in contacting us or your nearest distributor.
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| CUSTOM
DNA SEQUENCING |
| GENAXIS Biotechnology offers unique
state of the art custom DNA sequencing services. Results obtained
are of top quality owing to the expertise used in our production facility.
All projects are treated confidentially.
ORDERING
INFORMATION: To order our Sequencing services you
will find a special order
form.
Please pay attention to the pretended Primer- and Template-concentrations:
Plasmid >3 µg (200 ng/µl) Vector (Clonig site / Insert size)
Cosmid >9 µg (600 ng/µl) Standard-Primer (free of charge) PCR-fragment
>1 µg (50 ng/µl) for ~1000 bp PCR-fragment >0,5 µg (25 ng/µl)
for ~500 bp PCR-fragment >0,3 µg (20 ng/µl) for ~200 bp Primer
(each) 5 µl (10 pmol/µl) 18 - 25 mer
Send your DNA only dissolved in Water (not in buffer !) and use
the same name and marks on sample-tube and order-form.
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| FISH-AXIS:
Fluorescence in situ Hybridization services |
With GENAXIS Biotechnology, FISH is faster! We supply fast and reliable
human chromosomal localization from your own DNA clones: genomic DNA
or cDNA. These can be in the form of:
- PCR product
- BAC
- PAC
- YAC
- Plasmid
- Cosmid
For FISH-AXIS services please provide:
- cDNA or DNA clones > 5 Kb (for DNA fragments between 1 and
5 Kb please contact us)
- at least 5 - 10 µg of DNA (solubilized in water)
- the purification system you have used
- the concentration of the DNA supplied
- a picture of your DNA material after gel electrophoresis
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| Custom antibody
production |
| GENAXIS Biotechnology offers the fast and high quality production
of polyclonal antisera from your specific antigen. Antibody production
is performed on selected animals. Mouse, rat, rabbit, guinea pig,
cock-hen, sheep, goat are systematically available; horse, donkey
and pig are available on request.
Our prices include animal housing, food, immunisation, taking of
blood.
IMPORTANT: No extra cost for purchase of animal or its
monthly maintenance! Freight costs are not included in our pricing.
Send your antigen:
- lyophilized
- in sloution in physiological buffer (e.g. PBS buffer pH 7.0-8.0)
- included in a PAGE slice
- immobilised on a nitro-cellulose membrane.
GENAXIS Biotechnology offers the fast and high quality production
of polyclonal antisera from your specific antigen. Antibody production
is performed on selected animals. Mouse, rat, rabbit, guinea pig,
cock-hen, sheep, goat are systematically available; horse, donkey
and pig are available on request. Our prices include animal housing,
food, immunisation, taking of blood. IMPORTANT : No extra cost for
purchase of animal or its monthly maintenance! Freight costs are
not included in our pricing.
Send your antigen :-lyophilized-in solution in physiological buffer
(e.g. PBS buffer pH 7.0-8.0)-included in a PAGE slice-immobilised
on a nitro-cellulose membrane.
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| Custom peptide
antibody production |
| In this integral peptide antibody production GENAXIS Biotechnology
offers: synthesis of your peptide sequence (15 to 30 amino acids),
specific conjugation to carrier proteins (Bovine Serum Albumin, Ovalbumin
or Keyhole Limpet Hemacyanin), standard immunisation using a three
month program for two rabbits.
ALSO AVAILABLE:
- Peptide synthesis including peptide modifications, peptide conjugation
to carrier proteins.
- Antibody labelling: HRPeroxydase, Biotin, Fluorescence.
- Antibody purification: affinity purification using A or G Sapharose,
affinity purification of sera using the antigen matrix.
- Antibody fragmentation: fab-fragments SCS-PAGE or ELISA analysed.
Please do not hesitate contacting us for further technical assistance
or prices.
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